Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). 05 mg mL −1. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. Assessment of Neurite Outgrowth. e. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The IP-10 standard curve was prepared as described in Section 10. 2. 10. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). M2 M2e M3 M4 M5 M5e ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SPECTROMAXx with iCAL 2. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Following digestion, 200 µl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485 and 525 nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). , contact Molecular. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. Fluorescence Microplate Reader Comparison Chart. Background-subtracted data values (mean +/- SD) are graphed. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Italic and boldface type are used for emphasis. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. 1 mg·mL −1 (Sigma-Aldrich, USA) as described above and fluorescence intensity at λ ex = 530 nm and λ em = 590 nm was detected through a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA). Description 1. S. What types of plates can be used with StakMax? SBS Standard 96 and 384 well plates with plate height of 12-16 mm are recommended. With optimized reagents, option to operate the. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. SpectraMax® iD5. Luminescence was immediately measured by a SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA) after mixing the fecal supernatant and Hgb standard with the same amount of luminol reagent. 2. Detection modalities are shown in Table 1-1. D 1 1. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). RFU was plotted versus time to derive the rate of formation of the fluorophore. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. FlexStation™ III. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. All associated StakMax® stackers. To report an issue with this product, Brief content visible, double tap to read full content. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. We therefore gathered salient. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Each well was scanned at nine evenly distributed points and averaged automatically with the software. When a feature or capability applies to only certain readers, this exception is noted. 0. Background-subtracted data values (mean +/- SD) are graphed. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. , USA). SpectraMax® M5. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. C. Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Plate chamber design of the SpectraMax category 1 microplate readers. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. . Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Three kits covering a wide range of dsDNA concentrations. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Here, we present an approach to predict UV-visible absorption spectra of solvated aromatic molecules by quantum chemistry (QC) and machine learning (ML). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). Choose from a three- (M3), four- (M4), orCables. 2. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. SpectraMax Microplate Readers. Standard features include a cuvette port,. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. Background-subtracted data values (mean +/- SD) are graphed. After treatment, the Cell Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, Rockville, MD, USA) was added to the PA and skatole treated hepatocytes and additionally incubated for 1 h. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). The available phosphorus in the soil was leached by double-acid extraction (0. 次いで、ユーザーのラボに. Choose from a three- (M3), four- (M4), or Cables. Molecular Devices SpectraMax® series microplate readers. normalized response (Graphpad Prism 8. In. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax® M4). Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Click Read. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Acridine orange (AO) staining evaluates the morphological changes in apoptotic cells by formation of blebs in the membrane, fragmentation and condensation of the. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. All experiments were performed in triplicate. In vitro transcription (IVT) is an essential technique for RNA synthesis. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Background-subtracted data values (mean +/- SD) are graphed. 3. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. . The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities for Detection Estimates, Magnitude 1 through 6. The test conditions are as follows: mobile phase: acetonitrile/H2O (0. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Statistical analysis. Full content visible, double tap to read brief content. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). SpectraMax M4 Multi-Mode Microplate Reader. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. spr SpectraMax iD5 SpectraTest ABS2 v1. 116 SpectraMax M5 and M5e Multi-Mode. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. ® reader to the computer. Description 1. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 304 signal level saturation. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Polybead® nanoparticles were analyzed. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. B07F4876MS. Turn the instrument around so that the back of the instrument is. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. 2 only) SpectraMax M-series Microplate readers. of a 96 well-plate and cultured for 24h at 37 °C. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. 0. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. Spectramax M Series, supplied by Danaher Inc, used in various techniques. *Statistical significance: p < 0. Parameter SpectraMax iD5 SpectraMax i3x. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). This platform allows the. For SpectraMax L or LMax II, LMax II 384 - no adapter used. D 47 7. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 1step assay with SpectraMax microplate readers. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeThen, cells were stained with MitoSox Red (5 μM) for 30 min. However, little is known about the regulatory mechanisms of the degradative pathway. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. D 1 1. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Colony formation assays were performed to measure cell proliferation. 7°C and the bottom to 36. . FlexStation ®, SpectraMax. Instruments Ltd. Molecular Devices SpectraMax M4 User Manual (86 pages) Multi-Mode Microplate Readers. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. All of the experiments were performed in triplicate, and the results were represented as mean. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Interpolated concentrations of native CRP in human serum and plasma samples. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). 005) Photometric PrecisionThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. 1 Figure from 528 Publications Molecular Devices LLC. Background-subtracted data values (mean +/- SD) are graphed. l. • Tel. FlexStation™ II. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Plot the fluorescence intensity versus the cell seeding number. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. The following document is intended to demonstrate setup of this instrument. 1. ” The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Dettmer4, Wayne A. 1. Large Molecule Bioanalysis. The drawing shows a standard microplate of 15 mm height covered with a lid. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Detection modalities are shown in Table 1-1. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. Detection modalities are shown in Table 1-1. ROS estimation by 2′,7′- dichlorofluorescin diacetate (DCFDA) staining . Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Results were expressed as the mean percentage of CV compared with NC group. Table 1. Background-subtracted data values (mean +/- SD, n =2) are graphed. 38. Microplate Reader Shelf. On day 2, in the morning weight the mice. The following document is intended to demonstrate setup of this instrument. The percentage of viability of each treatment group in different. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. Recertification is recommended every two years. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. Optimized for fluorescence microplate readers. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers; Instrument Field Depot; Aquamax 2000 Aquamax 2000/Stakmax. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. . Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Description 1. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. 90-0083 series): Excitation 530 nm,. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. 7. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). com. Serial number location on Series II and Multi-Mode plate readers. 5. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. 1. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR-FRET、蛍光偏光アッセイにおいても専用機に匹敵す. SpectraMax® Paradigm® Multi-Mode Detection Platform. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. 383 inches whereas the maximum allowed is 5. org) with optimized instrument settings (Table 1) were used to generate and analyze data. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 2. Other technologies such as imaging. The data represent the mean ± SD of three independent experiments. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. However, traditional polyacrylamide gel electrophoresis (PAGE) and high. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. . All configurations. 1. All configurations. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. 0397 in x 3. 01) + 0. 2 only). $2,219. 2. Allows you to use EOS lenses on a micro four thirds camera M4/3 K&F concept lens adapter. 316 grating motor driver faulty. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. 3 or greater. , cyan fluorescence at 458 ex. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The combination index (CI) was calculated. FP assay do not perform well with protein:protein interactions. Dumont3, Matthias S. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). 305 reference level saturation. 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). Pentobarbital sodium and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. The activity of untreated cells was normalized to 100% and the treated cell activity was compared with that. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Using. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. 95. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). 3. August 16, 2017. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. 03 in x 3. The MTT reagent was incubated with cells for 4 h. Multi-Mode Microplate Readers. 1. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. SpectraMax® ABS Plus. . Description 1. NCI-60 Human Tumor Cell Line Screen. For heme titration experiments a constant ATP concentration of 1 mM was used. free. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Choose cables that meet the requirement described in this appendix. Vol 4, Iss 22, Nov 20, 2014 . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Description 1. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). 2. ® reader to the computer. The DQ Green BSA probe. MIC assay: After diluting colloidal nanoparticle solutions of varying concentrations in TSB, they were injected onto a 96-well plate with a strain of bacteria at a concentration of 10 6 CFU/mL. , USA). Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Example: [Drawer]. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The IC50 curves were calculated using variable slope for log (inhibitor) vs. , UK) or SpectraMax M4 by Molecular Device. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Highly selective for dsDNA over ssDNA or RNA. spr SpectraMax Plus 384. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. Titration of human serum and plasma (heparin) within the working range of the assay. , and red fluorescence at 543 ex. Ltd. The SpectraMax M5e reader offers the additional benefit of being certified . Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Page: 1/6. Briefly, 4T1 cells were seeded (5000 cells/well) in a 24-well plate. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. At sacrifice, serum was isolated from mouse total blood and maintained at –80 °C. SpectraMax® L (software version 5. Fluorescence Microplate Reader Comparison Chart. The absorbance of solubilized formazan crystals was measured at 570 nm using a multimode reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, CA, USA). View specifications, prices, citations, reviews, and more. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. SpectraMax Gemini XPS/EM Register or Sign In. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Note: In this user guide, all references to SpectraMax Multi-Mode. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Cellular viability was assessed by using resazurin at 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. (Shanghai, China). Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. overnight. Measurement of Mitochondrial Membrane Potential by JC-1. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). Then, MTT (10 μl of 5 mg/ml) was added to each well and further incubated for 4 h. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. 7. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). Background-subtracted data values (mean +/- SD) are graphed. SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Phillips5, Martin A. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Dettmer4, Wayne A. SpectraMax M3, M4, M5, and M5 e. Formazan crystals formed were solubilized in 10 μl DMSO, and absorbance was recorded using a multimode plate reader (SpectraMax M4; Molecular Devices, USA) at 606-nm wavelength. . 5. Louis, MO, USA). Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. Category 2 and 3 microplate readers use gradient heating as well.